After receival of the microalgae Chlorella Vulgaris in a 50 mL falcon and the cultivation of said microalgae in a 5-liter bioreactor lies a lot of obstacles and problems (Roughly 4 weeks). At first, the microalgae did not multiply as hoped, showing a lag-period in which, the microalgae adopted to our lab environment. Furthermore, we experienced a bottleneck in 500 mL culture flasks. After transfer of our culture in a 2 L bioreactor the hoped proliferation of the microalgae could not be seen. This however changed, after addition of fumigation with the air in our lab. In this way the microalgae could proliferate very quickly and in a secure and sterile way, so that we were able to transfer the microalgae from a 2 L bioreactor to a 5 L bioreactor, generating biomass able to be used in our experiments!
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